Reporter

Part:BBa_K895006:Design

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-23)

GFP open reading frame


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We moved the GFP gene from BBa_J54103 into pSB1C3 with the correct prefic and suffix sequences to bring it up to RFC[10] standard.

Source

Amplified by PCR from BBa_J54103

References